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Chèvre anti-Souris IgA (Fc Region) Anticorps

ELISA, WB, IF, DB Polyclonal unconjugated
N° du produit ABIN458505

Aperçu rapide pour Chèvre anti-Souris IgA (Fc Region) Anticorps (ABIN458505)

Antigène

IgA

Clonalité

  • 276
  • 67
Polyclonal

Application

  • 172
  • 133
  • 129
  • 98
  • 96
  • 86
  • 50
  • 46
  • 37
  • 33
  • 26
  • 22
  • 17
  • 16
  • 16
  • 14
  • 13
  • 7
  • 7
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Western Blotting (WB), Immunofluorescence (IF), Dot Blot (DB)
  • Épitope

    • 71
    • 40
    • 37
    • 18
    • 15
    • 13
    • 8
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Fc Region

    Reactivité

    • 171
    • 53
    • 38
    • 15
    • 14
    • 13
    • 13
    • 12
    • 12
    • 11
    • 9
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Souris

    Hôte

    • 179
    • 101
    • 53
    • 17
    • 14
    • 6
    • 2
    Chèvre

    Specificité

    IgG fraction of polyclonal Goat antiSerum to Mouse IgA, Fc specific

    Réactivité croisée (Details)

    Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this immunoconjugate has not been tested in detail.

    Attributs du produit

    The reactivity of the antiserum is directed to the Fc subunit of the IgA molecule which expresses strict isotypic (class) specificity. It does not react with IgG, IgM and IgG/Fab or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.

    Purification

    Purified

    Immunogène

    Purified homogenous IgA isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
  • Indications d'application

    Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

    Buffer

    Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

    Agent conservateur

    Without preservative

    Stock

    RT,4 °C,-20 °C

    Stockage commentaire

    The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • Antigène

    IgA

    Classe de substances

    Antibody
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